Gene expression of two innate cytokines in a miscarriage toxoplasmosis woman.

Abstract

Toxoplasma gondii infections are endemic in Iraq and represent serious problems. Human toxoplasmosis can be associated with serious clinical manifestations, particularly in developing fetus. This study was aimed to determine the distribution of genotypes and alleles, residing within interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) polymorphisms, among fetuses and neonates, congenitally infected with Toxoplasma gondii, and among uninfected control cases. Blood samples were collected from 125 aborted women with a history of single or recurrent miscarriage, in addition to fifty normal healthy control women. Molecular identification of the parasite was performed by detecting Toxoplasma B1 gene using real-time qPCR technique. IL-6 and IL-1β gene expression was assayed in each case-study samples by using RT-PCR. T. gondii was detected in recurrent toxoplasmosis aborted women at percent (16%). IL-1β and IL-6 gene expression was significantly increased in toxoplasmosis women compared with healthy control women. Fold expression of IL-1β was 9.5 in toxoplasmosis patients compared with one fold in healthy control. IL-1β and IL-6 over-expression was correlated to the high-risk toxoplasmosis infection and could be a biomarker for prognosis of the disease.