Gene Expression of Transient Receptor Potential Channels in Peripheral Blood Mononuclear Cells of Inflammatory Bowel Disease Patients.

Taku Morita,Takuji Torimura,Kotaro Kuwaki,Keiichi Mitsuyama,Atsushi Mori,Masaru Morita,Kozo Tsuruta,Sayo Yamasaki,Toshihiro Araki,Shinichiro Yoshioka,Tetsuhiro Yoshimura,Hidetoshi Takedatsu,Hiroshi Yamasaki

doi:10.3390/jcm9082643

Taku Morita, Takuji Torimura + Show 11 more

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https://doi.org/10.3390/jcm9082643

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Abstract

We examined the expression profile of transient receptor potential (TRP) channels in peripheral blood mononuclear cells (PBMCs) from patients with inflammatory bowel disease (IBD). PBMCs were obtained from 41 ulcerative colitis (UC) patients, 34 Crohn’s disease (CD) patients, and 30 normal subjects. mRNA levels of TRP channels were measured using the quantitative real-time polymerase chain reaction, and correlation tests with disease ranking, as well as laboratory parameters, were performed. Compared with controls, TRPV2 and TRPC1 mRNA expression was lower, while that of TRPM2, was higher in PBMCs of UC and CD patients. Moreover, TRPV3 mRNA expression was lower, while that of TRPV4 was higher in CD patients. TRPC6 mRNA expression was higher in patients with CD than in patients with UC. There was also a tendency for the expression of TRPV2 mRNA to be negatively correlated with disease activity in patients with UC and CD, while that of TRPM4 mRNA was negatively correlated with disease activity only in patients with UC. PBMCs from patients with IBD exhibited varying mRNA expression levels of TRP channel members, which may play an important role in the progression of IBD.

Highlights

Inflammatory bowel disease (IBD), consisting primarily of ulcerative colitis (UC) and Crohn’s disease (CD), represents a group of chronic inflammatory disorders involving the gastrointestinal tract
Saunders et al examined the expression of TRPV1 and TRPV2 in peripheral blood mononuclear cells (PBMCs) from healthy subjects and speculated that as they have a role in the detection of noxious stimuli in the blood or under pathological conditions, their upregulation acts as an indicator of inflammation [13,21]
To the best of our knowledge, the current study is the first to explore the relationships among the gene expression profiles of transient receptor potential (TRP) channel members in PBMCs from patients with IBD

Summary

Introduction

Inflammatory bowel disease (IBD), consisting primarily of ulcerative colitis (UC) and Crohn’s disease (CD), represents a group of chronic inflammatory disorders involving the gastrointestinal tract. The transient receptor potential (TRP) channel family, a diverse family of proteins that are reportedly expressed throughout the human body, have emerged as a novel and interrelated system to detect and respond to various environmental stimuli including mechanical, thermal, or chemical stimuli. With this function, they are likely to be sensors for monitoring specific responses to different exogenous and endogenous chemical and physical stimuli [3,4,5,6]. A vital interplay between these cells appears to maintain homeostasis in the intestine, while the disruption of this interplay may be involved in the development and maintenance of IBD [7,8,9,10]

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