Abstract
Background: N-heptyl-D-galactonamide (GalC7) is a small synthetic carbohydrate derivative that forms a biocompatible supramolecular hydrogel. In this study, the objective was to analyze more in-depth how neural cells differentiate in contact with GalC7. Method: Direct (ex vivo) cells of the fresh hippocampus and culture (In vitro) of the primary cells were investigated. In vitro, investigation performed under three conditions: on culture in neurospheres for 19 days, on culture in GalC7 gel for 7 days, and on culture in both neurospheres and GalC7 gel. Total RNA was isolated with TRIzol from each group, Sox8, Sox9, Sox10, Dcx, and Neurod1 expression levels were measured by qPCR. Result: Sox8 and Sox10, oligodendrocyte markers, and Sox9, an astrocyte marker, were expressed at a much higher level after 7 days of culture in GalC7 hydrogel compared to all other conditions. Dcx, a marker of neurogenesis, and Neurod1, a marker of neuronal differentiation, were expressed at better levels in the GalC7 gel culture compared to the neurosphere. Conclusions: These results show that the GalC7 hydrogel brings different and interesting conditions for inducing the differentiation and maturation of neural progenitor cells compared with polymer-based scaffolds or cell-only conditions. The differences observed open new perspectives in tissue engineering, induction, and transcript analysis.
Highlights
In vitro conditions that reproduce in vivo profile of gene expression is a critical foundation of cell culture
The Ct value of this mixture was determined, and we found that the beta-actin Ct value was unchanged. These results demonstrated that GalC7 gel did not inhibit PCR
This study shows that functional hippocampal cells from mouse embryos (E19.5) can be maintained ex vivo for several days on a supramolecular low molecular weight hydrogel based on a single small molecule, N-heptyl-D-galactonamide (GalC7)
Summary
In vitro conditions that reproduce in vivo profile of gene expression is a critical foundation of cell culture. Frustration in this area is high and there is a growing concern for research in particular, given the impact of working on living organisms and on the understanding of the mechanisms involved. Result: Sox and Sox, oligodendrocyte markers, and Sox, an astrocyte marker, were expressed at a much higher level after 7 days of culture in GalC7 hydrogel compared to all other conditions. A marker of neurogenesis, and Neurod, a marker of neuronal differentiation, were expressed at better levels in the GalC7 gel culture compared to the neurosphere. The differences observed open new perspectives in tissue engineering, induction, and transcript analysis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
