Abstract
Four isoforms of H+-ATPase of plasma membrane: MHA1, MHA2, MHA3, MHA4 are expressed in the corn seedling roots with prevalence of genes MHA3 і MHA4. The exposure of seedlings in the presence of 0.1 M NaCl activated the expression of MHA4 gene isoform, that demonstrates its important role in the processes of adaptation to salinization conditions. In vacuolar membrane, where potential is created by two Н+-pumps, sodium ions activated gene expression of only Н+-АТРase of V-type, taking no effect on the expression of Н+-pyrophosphatase. The seeds pretreatment by synthetic preparations Methyure and Ivine did not affect gene expression of Н+-pumps. Thus we can suppose that the ability of the above preparations to activate functioning of Н+-pumps in the presence of sodium ions is realized at the post-tranlation level.
Highlights
R epresentatives of the animal and plant kingdoms of nature are cardinally different as to their response to the ions of sodium which is vital for the former ones but unnecessary for others; in high concentrations it becomes a toxic element that is displayed at a cell level
Differences as to Na+ role in these organisms begin from the plasma membrane, since its electrochemical potential is created in the animal cell by electrogenic Na+-pump – Na+/K+-АТРase, and in plant cells – by electrogenic Н+-pump – Н+-АТРase [2], the both enzymes belonging to Е1-Е2-type of transport АТРase
Electrochemical potential on the vacuolar membrane is created by two Н+-pumps, the Na+ presence activating the functioning of only weaker pump, Н+АТРаse of vacuolar V-type, and taking no effect on gene expression of its subunit A and content of enzymatic protein [5, 7]
Summary
R epresentatives of the animal and plant kingdoms of nature are cardinally different as to their response to the ions of sodium which is vital for the former ones but unnecessary for others; in high concentrations it becomes a toxic element that is displayed at a cell level. Differences as to Na+ role in these organisms begin from the plasma membrane, since its electrochemical potential is created in the animal cell by electrogenic Na+-pump – Na+/K+-АТРase, and in plant cells – by electrogenic Н+-pump – Н+-АТРase [2], the both enzymes belonging to Е1-Е2-type of transport АТРase. It plays the important role in numerous physiological processes: opening and closing of stomata, osmoregulation, absorption of nutrients, root growth, adaptation to abiotic stresses, salinization conditions in particular [1]. The elucidation of mechanisms of salt-protective action of Methyure and Ivine, which we have performed earlier, has revealed the ability of Methyure to increase considerably transport activity of Н+-АТРases and functioning of Na+/Н+-antiporters in the plasma and vacuolar membranes of root cells [5, 10]
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