Gene Expression of Formyl Peptide Receptor 1 in Eosinophils of Iraqi Patients with Extrinsic Allergic Asthma

Abstract

The formyl peptide receptor 1 (FPR1) is a member of the seven transmembrane G-protein coupled receptors family, mainly expressed by polymorphonuclear and mononuclear phagocytes and play a role in chemotaxis, killing of microorganisms through phagocytosis, and plays a important role players in innate immunity and host defense. The expression of (FPR1) in eosinophils has been investigated intensively with many diseases. In this study we explain regulation of (FPR1) expression in eosinophils during allergic disease such as (extrinsic Asthma). The study were conducted by using Reverse Transcription Real-Time PCR technique, and the fold changes of (FPR1) were estimated in the house dust mite (HDM) hypersensitive asthmatic patients group and birch pollen hypersensitive asthmatic patients group as well as healthy control group. The results show that there are high significant increase fold changes of (FPR1) expression (up regulation) in eosinophils of HDM hypersensitive asthmatic patients group. In contrast, birch pollen hypersensitive asthmatic patients group have no significant increase in FPR1 expression when compared with healthy control group. In conclusion, we concluded that formyl peptide receptor 1 (FPR1) that expressed by eosinophils have potential role in immune regulation and allergic diseases mainly extrinsic asthma when patients exposed to exogenous aggressive factor such as house dust mite.