Gene expression of ECV304 cells after treated with endostatin detected by microarray analysis

Abstract

Objective To observe the altered gene expression of ECV304 cell after treated with endostatin (ES) using cDNA microarray, and to investigate its molecular mechanism. Methods BiostarH40S gene expression DNA microarrays were used to profile changes in gene expression of ECV304 cells after treated with ES for 36 hours. To prepare the probes, mRNA from both control and treated cells were isolated and purified, and then reversely transcribed to cDNA with the incorporation of fluorescent-labeled dUTP: Cy3 and Cy5 respectively. The probes were hybridized with expressed cDNA microarray, the fluorescent signals of Cy3 and Cy5 were scanned and analyzed by a computer system. Results ES inhibited the proliferation of ECV304 cells. The result of electrophoresis indicated that the extracted total RNA had high quality. Approximately 5. 96% of all human genes examined on 4096 gene microarrays showed altered expression, with 225 down-regulated genes and 19 up-regulated genes. Conclusion The expression of some genes of ECV304 cells decrease after treated with ES, which may be related to the mechanism of inhibition of neovascularization. Key words: Gene expression profiling/utilization; Microchip analytical procedures/method; Endostatins/pharmacology; Gene expression