Gene expression of cell surface antigens in the early phase of murine influenza pneumonia determined by a cDNA expression array technique.

Shinya Sakai,Naoki Mantani,Katsutoshi Terasawa,Hiroshi Ochiai,Toshiaki Kogure,Yutaka Shimada

doi:10.1080/0962935021000051557

Abstract

BACKGROUND: Influenza virus is a worldwide health problem with significant economic consequences. To study the gene expression pattern induced by influenza virus infection, it is useful to reveal the pathogenesis of influenza virus infection; but this has not been well examined, especially in vivo study. AIMS: To assess the influence of influenza virus infection on gene expression in mice, mRNA levels in the lung and tracheal tissue 48 h after infection were investigated by cDNA array analysis. METHODS: Four-week-old outbred, specific pathogen free strain, ICR female mice were infected by intra-nasal inoculation of a virus solution under ether anesthesia. The mice were sacrificed 48 h after infection and the tracheas and lungs were removed. To determine gene expression, the membrane-based microtechnique with an Atlas cDNA expression array (mouse 1.2 array II) was performed in accordance with the manual provided. RESULTS AND CONCLUSIONS: We focused on the expression of 46 mRNAs for cell surface antigens. Of these 46 mRNAs that we examined, four (CD1d2 antigen, CD39 antigen-like 1, CD39 antigen-like 3, CD68 antigen) were up-regulated and one (CD36 antigen) was down-regulated. Although further studies are required, these data suggest that these molecules play an important role in influenza virus infection, especially the phase before specific immunity.

Highlights

Influenza is a major epidemic viral disease in humans
The influenza virus interacts with cells on the luminal side of the airways and alveoli to induce the release of immunoactive mediators, initiating a cascade of events resulting in the eventual elimination of the virus
Cell surface antigens are expressed on the cell surface and play important roles in cell-cell interactions in various situations involving the site of influenza virus infection

Summary

Introduction

Influenza is a major epidemic viral disease in humans. Its continuing importance lies in its ability to cause pneumonitis, which occurs in previously fit individuals and in the elderly. The infection of mice with mouse-adapted influenza virus strains by aerosol inoculation results in severe disease with alveolar involvement. Little information has been gathered about the early phase of this response, before the appearance of specific cytotoxic T cells During this phase, the influenza virus interacts with cells on the luminal side of the airways and alveoli to induce the release of immunoactive mediators, initiating a cascade of events resulting in the eventual elimination of the virus. Cell surface antigens are expressed on the cell surface and play important roles in cell-cell interactions in various situations involving the site of influenza virus infection. Examining the expression of cell surface antigens in influenza virus infection contributes important information about the network among various cells. Further studies are required, these data suggest that these molecules play an important role in influenza virus infection, especially the phase before specific immunity

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Results