Gene Expression of Antioxidant Enzymes in the Resected Intestine in Crohn's Disease.

Otakar Sotona,Jan Bureš,Jiří Páral,Július Örhalmi,Tomáš Dušek,Alena Mrkvicová,Petr Lochman,Ondřej Malý,Veronika Knoblochová,Eva Peterová

doi:10.14712/18059694.2021.26

Abstract

The inflammatory process in Crohn's disease (CD) is closely associated with the formation of reactive oxygen species. Antioxidant enzymes can play an important role in the outcome of CD and may influence postoperative recurrence in these patients. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. A total of 28 patients referred for elective bowel resection were enrolled in the study. Full-thickness small intestinal specimens were investigated. Gene expression of antioxidant enzymes - superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) - was evaluated both in macroscopically normal and inflamed samples. There were significantly lower levels of SOD1 mRNA (p = 0.007) and GSR mRNA (p = 0.027) in inflamed tissue compared to macroscopically normal areas. No significant differences were found between affected and non-affected intestinal segments in mRNA for SOD2, SOD3 and GPX. Our pilot data clearly showed that the gene expression of major antioxidant enzymes is not a uniform mechanism in the pathogenesis of Crohn's disease. Topically decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species.

Highlights

The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species
The mRNA for each enzyme in the non-pathological and pathological tissue of the males group was compared to the mRNA for each enzyme in females group
The mRNA for each enzyme in the non-pathological and pathological tissue of the smokers was compared to the mRNA for each enzyme in non-smokers

Summary

Introduction

The inflammatory process in Crohn’s disease (CD) is closely associated with the formation of reactive oxygen species. The aim of our study was to evaluate gene expression of intracellular antioxidant enzymes in surgically resected intestinal specimens of patients with CD, both in macroscopically normal and in inflamed tissue. Gene expression of antioxidant enzymes – superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GSR) – was evaluated both in macroscopically normal and inflamed samples. Decreased gene expression of SOD1 and GSR might facilitate the segmental tissue injury caused by reactive oxygen species. Ulcerative colitis affects the large bowel only, whereas CD can involve any part of the gastrointestinal tract (most commonly the ileum and proximal colon). These diseases have somewhat different pathologic and clinical characteristics, but with substantial overlap. Either too strong or inadequate immune response to microbes in the intestinal lumen can result in intestinal inflammation [3]

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