Abstract
Leptospira genus contains species that affect human health with varying degrees of pathogenicity. In this context, we aimed to evaluate the differences in the modulation of host gene expression by strains of Leptospira varying in virulence. Our data showed a high number of differentially expressed transcripts in murine macrophages following 6h of infection. Leptospira infection modulated a set of genes independently of their degree of virulence. However, pathway analysis indicated that Apoptosis, ATM Signaling, and Cell Cycle: G2/M DNA Damage Checkpoint Regulation were exclusively regulated following infection with the virulent strain. Taken together, results demonstrated that species and virulence play a role during host response to Leptospira spp in murine macrophages, which could contribute to understanding the pathogenesis of leptospirosis.
Highlights
Leptospirosis disease can occur in different epidemiological conditions [1]
Microarray raw data files are available in Gene Expression Omnibus (GEO) and are accessible through GEO series number GSE105141 [22]
Through treatment comparison by Venn diagram, we identify common and specific genes (Fig 2)
Summary
The genus Leptospira encompasses pathogenic and saprophytic species that differ in their ability to survive and colonize different environments and hosts [2]. Leptospirosis occurs mainly in vulnerable populations, including urban and rural dwellers [4] of tropical and subtropical developing countries [5,6,7]. It is a major public health problem, with a recent estimate of 1 million cases per year, and a mortality rate of 5 to 10% [4,8,9]. Transmission is based on direct or indirect contact with the urine of carriers (mainly rodents); the disease varied from sub-clinical to most serious cases, progressing to renal failure and pulmonary
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