Abstract
We investigated gene expression responding to hydrodynamic stress in an agitation tank using RT-PCR. A novel fragment, tuf, was found to be expressed under turbulent flow conditions using a differential display method. The deduced amino acid sequence of the fragment was homologous to those of NBS (nucleotide binding site) domains in plant disease-resistant genes, but differed with respect to some of the functional motifs of those sequences. We also demonstrated that calcium-dependent protein kinase (CDPK) expression increased by agitation. A calcium channel blocker, verapamil, suppressed the expression of tuf, but did not affect the expression of CDPK.
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