Abstract
Abstract We constructed two inducible plasmid vectors which were different at the replication origin to control the expression of heterologous genes in Bacillus brevis. The Escherichia coli lac repressor gene was expressed by using the promoter region of the cell wall protein (MWP) gene of B. brevis 47. The lac operator was placed downstream from the P2 promoter of the MWP gene. The gene of Bacillus licheniformis α-amylase (BLA) or for human salivary α-amylase (HAMY) was inserted immediately downstream of the lac operator. The production of BLA by B. brevis carrying the inducible plasmids pNU550BLA and pHT550BLA thus constructed was induced 8 and 100-fold, respectively, by the addition of isopropyl-β- d -thio-galactopyranoside (IPTG). The production of HAMY carrying the inducible plasmid pNU550HAMY was induced 6-fold by the addition of IPTG.
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