Gene expression changes in Maconellicoccus hirsutus in response to sublethal dose of Buprofezin

Abstract

The pink or hibiscus mealybug, Maconellicoccus hirsutus, is a serious pest of grapes, jute, and mesta, causing severe yield losses in India and other countries. Chemical control remains the foremost choice for farmers to manage this pest. As insecticides break down over time due to biotic and abiotic factors, insects are exposed to varying levels of these exogenous compounds. Several studies have reported that sublethal doses affect insect physiology, but only a few have examined the changes in gene expression at the molecular level. Therefore, studies were conducted to elucidate the molecular mechanisms in M. hirsutus exposed to sublethal doses of buprofezin 25 SC. Life table analysis revealed increased fecundity in M. hirsutus exposed to the sublethal dose. A total of 1,744 differentially expressed genes were identified between the buprofezin-treated and untreated samples using transcriptome analysis. These genes were primarily associated with ribosomal proteins, proteases, cuticular proteins, and cytoskeletal structures. Ribosomes and phagosomes were the most highly enriched pathways. Interestingly, most of the DEGs were involved in restoring homeostasis rather than detoxification. To validate our RNA-sequencing results, qRT-PCR validation was performed on ten randomly selected genes. Overall, our findings provide valuable insights into intermittent changes in stress-coping genes, apart from detoxification genes.