Gene expression and localization of arabinogalactan proteins during the development of anther, ovule, and embryo in rice.

Abstract

Arabinogalactan proteins (AGPs) are hyperglycosylated members of the hydroxyproline-rich glycoprotein (HRGP) superfamily and are widely distributed throughout the plant kingdom. In Oryza sativa (rice), the gene expression and biological function of AGPs only have received minimal research attention. Here, we used qRT-PCR to detect the expression patterns of OsAGP genes in various organs, and found that six genes were preferentially expressed in panicles, three genes were specifically expressed in anthers, and one gene in the stigma. Furthermore, using four specific monoclonal antibodies (JIM8, JIM13, LM2, MAC207), we observed the distribution of AGPs in rice anthers, ovules, and embryos. In anthers, the strong fluorescence signals of AGPs were present in tapetum cells, pollen mother cells, and mature pollens, suggesting that AGPs might be related to the development of anther and pollen. In ovules, signals of AGPs were specifically distributed in the three micropylar megaspores of the tetrad, and with intense signals in the egg cell and synergid cells in the mature embryo sac. This suggests that AGPs may be involved in megaspore determination and double fertilization. In embryos, the immunological signals of AGPs appeared in peripheral and inner cells at the early stage, and in the scutellum, plumule, and radicle at the late stage, indicating that AGPs may be associated with organ differentiation and maturation of embryos. In this study, we revealed that AGPs were widely distributed in rice anthers, ovules, and embryos, which lays a foundation for the functional investigation of AGPs in various processes of sexual reproduction.