Abstract
Potential applications for gene-based tissue engineering therapies in the oral and maxillofacial complex include the delivery of growth factors for periodontal regeneration, pulp capping/dentin regeneration, and bone grafting of large osseous defects in dental and craniofacial reconstruction.Part 1 reviewed the principals of gene-enhanced tissue engineering and the techniques of introducing DNA into cells. This manuscript will review recent advances in gene-based therapies for dental hard tissue regeneration, specifically as it pertains to dentin regeneration/pulp capping and periodontal regeneration.
Highlights
The goal of gene-enhanced tissue engineering is to regenerate lost tissue by the local delivery of cells that have been genetically-enhanced to deliver physiologic levels of specific growth factors. The basis for this approach lies in the presence of a population of progenitor cells that can be induced, under the influence of these growth factors, to differentiate into the specific cells required for tissue regeneration, with guidance from local cues in the wound environment [1]
Reconstruction, and articular cartilage repair [3,4]. This manuscript will review recent advances in gene-based therapies for dental hard tissue regeneration, as it pertains to dentin regeneration/pulp capping and periodontal regeneration
From a tissue engineering approach, the oral cavity has significant advantages compared to other sites in the body, including easy access and observability
Summary
BMP-7 gene transfer to inflamed ferret dental pulps. Eur J Oral Sci 2001, 109:422-424. Shi S, Bartold P, Miura M, Seo B, Robey P, Gronthos S: The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Iohara K, Makashima M, Ito M, Ishikawa M, Nakasima A, Akamine A: Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2. Nakashima M, Mizunuma K, Murakami T, Akamine A: Induction of dental pulp stem cell differentiation into odontoblasts by electroporation-mediated gene delivery of growth/differentiation factor 11 (Gdf). Nakashima M, Iohara K, Ishikawa M, Ito M, Tomokiyo A, Tanaka T, Akamine A: Stimulation of reparative dentin formation by ex vivo gene therapy using dental pulp stem cells electrotransfected with growth/differentiation factor 11 (Gdf). Goldberg M, Smith AJ: Cells and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering.
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