Gene effects and mean performance of nitrogen and phosphorus use in wheat after inoculation with arbuscular mycorrhiza fungi and Azotobacter chroococcum under low input conditions

Abstract

The present investigation was conducted to study the impact of bio-inoculants under low input field conditions on the magnitude and direction of gene effects and the mean performance of nitrogen (N) and phosphorus (P) use in wheat. Three wheat cultivars suitable for different agro-ecological conditions, i.e. WH 147 (low mineral input), WH 533 (water deficit), Raj 3077 (high mineral input), and six generations (P1, P2, F1, F2, BC1and BC2) of three crosses, namely WH 147 × WH 533, WH 533 × Raj 3077 and WH 147 × Raj 3077, were evaluated in a randomized block design with three replications under low input field conditions (80 kg N + 40 kg P + 18 kg ZnSO4doses applied in each treatment) with three treatments, i.e. control, inoculation with arbuscular mycorrhiza fungi (AMF,Glomus fasciculatum) and dual inoculation with AMF andAzotobacter chroococcum(Azc). Bioinoculation with AMF and AMF+Azchad a positive impact on the mean performance of all the wheat crosses. The mean performance of AMF was maximum in the cross WH 147 × WH 533 for N and P response (%), N and P use index (%) and P content (ppm), whereas for N and P uptake it was maximum in the cross WH 147 × Raj 3077. The response and use index for N and P were better in the combined AMF+Azctreatment in all three crosses. The adequacy of the additive-dominance model for the phosphorus uptake (mg/plant) by all three crosses in all three treatments (i.e. control, AMF, AMF+Azc) suggested that additive (d) and dominance (h) gene effects mainly governed the inheritance of this trait. In all cases, digenic interactions were present, where the duplicate type of epistasis prevailed except for the P content in the control in the cross WH 147 × WH 533, where the complementary type of interaction was present. Pedigree selection in crosses WH 147 × WH 533 and WH 147 × Raj 3077 could be effective for breeding pure lines of wheat for sustainable agriculture (low input genotypes responsive to biofertilizers such as AMF andAzotobacter).