Abstract
The application of gene editing (GE) technology to create precise changes to the genome of bird species will provide new and exciting opportunities for the biomedical, agricultural and biotechnology industries, as well as providing new approaches for producing research models. Recent advances in modifying both the somatic and germ cell lineages in chicken indicate that this species, and conceivably soon other avian species, has joined a growing number of model organisms in the gene editing revolution.
Highlights
(Salter et al 1987; Bosselman et al 1989; Page et al 1991; McGrew et al 2004; Lee et al 2007)
By fusing the DNA-cleavage domain of FokI onto a TALE, TALE nucleases (TALENs) were produced and shown to be applicable to gene editing outside their native plant-host system for generating genetic changes by both non-homologous end joining (NHEJ) and homology-directed repair (HDR) (Fig. 1) (Miller et al 2011; Wood et al 2011)
CRISPR/Cas9 is a quick and flexible tool for the recognition and cutting of specific genomic sites, it is met with similar problems encountered by TALENs when applied to avian germline transgenesis
Summary
(Salter et al 1987; Bosselman et al 1989; Page et al 1991; McGrew et al 2004; Lee et al 2007). Most genome editing studies reported so far for birds have described the application of CRISPR/Cas9 to avian somatic cells and tissues.
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