Gene editing (CRISPR-Cas) technology and fisheries sector

Abstract

Considering the advantages of gene editing technologies, in recent years, emphasis has been given on three main techniques of gene editing i.e. zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 RNA-guided endonuclease system. In all of these three technologies one thing is common i.e. the technology utilizes restriction enzymes to break down the double stranded DNA molecule at a targeted location with the help of another molecule of homologous binding protein or RNA, which is also called as a guide RNA molecule. This targeted breaking and repair of the DNA molecule as per our requirement is generally viewed as a great breakthrough in gene therapy methods. In fisheries sector, in order to promote aquaculture/mariculture activities, aquaculture industries are facing number of challenges, particularly in area of quality and demand of seed production, control of health and disease management, production of quality traits with phenotypically improved varieties, and strengthening of immune system. Several efforts are being made both at public and private sectors to develop scientific technologies to meet these challenges and substantial achievements have also been made. But still these challenges remained as major constraints in hampering the growth of this industry as per the demand and expectations. However, with the advent of now recently developed gene editing techniques, we may have to explore and evaluate the possibilities of applications of this technology as a long lasting solution in addressing at least some of the vital issues of the aquaculture industry particularly in those related to altering targeted gene structure in the species for positive impact.