GENE DIVERSITY IN GREVILLEA POPULATIONS INTRODUCED IN BRAZIL AND ITS IMPLICATION ON MANAGEMENT OF GENETIC RESOURCES

Valderês Aparecida De Sousa,Fernando Albertin,Antonio Nascim Kalil Filho,Emerson Gonçalves Martins,Jarbas Yukio Shimizu

doi:10.1590/1806-90882018000200005

Valderês Aparecida De Sousa, Fernando Albertin + Show 3 more

Open Access

https://doi.org/10.1590/1806-90882018000200005

Copy DOI

Abstract

ABSTRACT We describe isoenzymes variability in six populations of Grevillea robusta from a provenances and progenies test established in a randomized block design with five plants per replication in Southern Brazil. The population genetic structure was examined by using biochemical markers in 5-year old trees, specifically at MDH-3, PGM-2, DIA-2, PO-1, PO-2, SOD-1, and SKDH-1 loci. The northern provenances (Rathdowney and Woodenbong) showed a strong divergence in relation to the average of provenances when alleles per locus (Ap), allele richness (Rs), Nei's gene diversity (H), and inbreeding coefficient (f) were considered. Inbreeding in varying degrees was detected. The commercial control showed the highest inbreeding coefficient, (f = 0.4448), whereas the provenance average was f = 0.2306, possibly due to insufficient sampling of populations in their origin (Australia). Despite its restricted natural range, a positive correlation between genetic divergence and geographic distance among original populations was detected. Genetic distance and cluster analyses based on the Bayesian model revealed three distinct provenance groups: 1) Rathdowney-QLD and Woodenbong-QLD; 2) Paddy's Flat-NSW; and 3) Mann River-NSW, Boyd River-NSW and the commercial control (material used in Brazil). The grouping of the control to both Mann River-NSW and Boyd River-NSW provenances suggests that the northern provenances have the highest potential for genetic improvement of wood productivity in Brazil, due to their high genetic diversity and low inbreeding coefficient.

Highlights

We describe isoenzymes variability in six populations of Grevillea robusta from a provenances and progenies test established in a randomized block design with five plants per replication in Southern Brazil
Genetic distance and cluster analyses based on the Bayesian model revealed three distinct provenance groups: 1) Rathdowney-QLD and Woodenbong-QLD; 2) Paddy’s Flat-NSW; and 3) Mann River-NSW, Boyd River-NSW and the commercial control
Orara River is a tributary of Clarence River that runs mostly through New South Wales at altitudes varying from sea level to 1,100 m at Bunya Mountains in Queensland (Harwood et al, 1997). This species occurs in two main habitats: in narrow bands associated with Eucalyptus along Richmond and Clarence rivers, and mixed with Araucaria cunninghamii Aiton ex

Summary

Introduction

Orara River is a tributary of Clarence River that runs mostly through New South Wales at altitudes varying from sea level to 1,100 m at Bunya Mountains in Queensland (Harwood et al, 1997) This species occurs in two main habitats: in narrow bands associated with Eucalyptus along Richmond and Clarence rivers, and mixed with Araucaria cunninghamii Aiton ex. According to a survey by Harwood (1992), grevillea is naturally distributed in a number of small populations distant two to 10 km apart. This situation leads to genetic structures with strong differentiation among and weak within populations (Moran and Hoper, 1983, 1992)

Methods

Results

Discussion

Conclusion