Abstract
A soilborne Stenotrophomonas sp. strain (MA5) that is resistant to mercury was isolated. A draft genome sequence-based analysis revealed a suite of gene determinants to resist mercury and other heavy metals, multidrug efflux, stress response, and membrane transport, and these provide cues to a suite of mechanisms that underpin cellular survival in contaminated soil.
Highlights
A soilborne Stenotrophomonas sp. strain (MA5) that is resistant to mercury was isolated
Genome de novo assembly was performed with CLC Genomics Workbench (v11.0.1; Qiagen, Aarhus, Denmark), and sequences were trimmed with a quality threshold of Q20 and a requirement of 50 bases after trimming
Further genome mining revealed possible resistance mechanisms against Hg, which included the presence of merA, which encodes the enzyme mercuric reductase (MerA); the periplasmic Hg2ϩ-scavenging protein (MerP); and the inner membrane-spanning proteins (MerT and MerE), which are engaged in the transport of Hg2ϩ to the cytoplasm and its reduction by the activity of the MerA enzyme
Summary
A soilborne Stenotrophomonas sp. strain (MA5) that is resistant to mercury was isolated. To gain a deeper understanding into the genomic underpinings of Hg cycling, a single colony of strain MA5 was picked from a LBϩHg plate and inoculated into liquid LB medium and grown at 30°C in a shaker. DNA was extracted with Qiagen’s DNeasy PowerLyzer kit and sequenced with an Illumina HiSeq 2000 instrument (8).
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