Abstract
Key message Gene-based SNPs were identified and mapped in pea using five recombinant inbred line populations segregating for traits of agronomic importance. Pea (Pisum sativum L.) is one of the world’s oldest domesticated crops and has been a model system in plant biology and genetics since the work of Gregor Mendel. Pea is the second most widely grown pulse crop in the world following common bean. The importance of pea as a food crop is growing due to its combination of moderate protein concentration, slowly digestible starch, high dietary fiber concentration, and its richness in micronutrients; however, pea has lagged behind other major crops in harnessing recent advances in molecular biology, genomics and bioinformatics, partly due to its large genome size with a large proportion of repetitive sequence, and to the relatively limited investment in research in this crop globally. The objective of this research was the development of a genome-wide transcriptome-based pea single-nucleotide polymorphism (SNP) marker platform using next-generation sequencing technology. A total of 1,536 polymorphic SNP loci selected from over 20,000 non-redundant SNPs identified using deep transcriptome sequencing of eight diverse Pisum accessions were used for genotyping in five RIL populations using an Illumina GoldenGate assay. The first high-density pea SNP map defining all seven linkage groups was generated by integrating with previously published anchor markers. Syntenic relationships of this map with the model legume Medicago truncatula and lentil (Lens culinaris Medik.) maps were established. The genic SNP map establishes a foundation for future molecular breeding efforts by enabling both the identification and tracking of introgression of genomic regions harbouring QTLs related to agronomic and seed quality traits.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-014-2375-y) contains supplementary material, which is available to authorized users.
Highlights
IntroductionBeing a rich source of protein, polysaccharides and providing slowly digestible starch, soluble sugars, fiber, minerals, and vitamins, pea plays an important role in meeting human nutritional needs (Smýkal et al 2012)
Pea (Pisum sativum L., 2n = 2x = 14) belongs to the Fabaceae family of flowering plants which includes other important agricultural crops such as soybean, chickpea, lentil, alfalfa, peanut, and common bean
Information relating to the orthologous genes in the model is important since it enables an inference of the genomic position of contigs within the P. sativum genome, and aids in the selection of an optimal set of evenly distributed single-nucleotide polymorphism (SNP) markers
Summary
Being a rich source of protein, polysaccharides and providing slowly digestible starch, soluble sugars, fiber, minerals, and vitamins, pea plays an important role in meeting human nutritional needs (Smýkal et al 2012). Theor Appl Genet (2014) 127:2225–2241 mature seeds for food and feed, the pea crop can be used as a vegetable, forage, silage, or green manure. Pea contributes to the development of low-input farming systems by fixing atmospheric nitrogen, and serves as a break crop which reduces the need for pesticide inputs. Global dry pea cultivation over the past decade has ranged from 6.0 to 6.5 million hectares producing 9.4–11.7 million tons per year (FAOSTAT 2013). Global cultivation of vegetable pea has ranged from 1.6 to 2.2 million hectares producing 12.0–17.4 million tons per year over the past decade (FAOSTAT 2013)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
