Abstract
Our previous work demonstrated cancer‐testis (CT) genes as a new source of candidate driver of cancer. Recently, mounting evidence indicates that long noncoding RNAs (lncRNAs) with CT expression pattern could play a pivotal role in cancer biology. Here, we characterized a conserved CT long noncoding RNA (CT‐lncRNA), PCAT6, which is expressed exclusively in the testis and is reactivated in liver hepatocellular carcinoma (LIHC) tissues due to the highly frequent amplification. The expression in LIHC was correlated with clinical prognosis in TCGA data. Knockdown of PCAT6 could inhibit cell proliferation and migration in hepatocellular carcinoma (LIHC) cells. Gene set enrichment analysis (GSEA) based on coexpression network revealed that PCAT6 was involved in similar cilium‐related pathways in the testis and LIHC tissues. However, PCAT6 was mainly positively correlated with gametogenesis‐related pathways in the testis but was coexpressed with mitotic cell cycle genes in LIHC. Together, our data demonstrated that CT‐lncRNA PCAT6 represents the similarity and difference between tumorigenesis and gametogenesis. The CT expression pattern and important role in LIHC oncogenesis make PCAT6 an ideal target for LIHC diagnosis and therapy.
Highlights
Spearman rank sum test was used to evaluate the association of expression level with the copy number of prostate cancer‐associated transcript 6 (PCAT6) in 364 liver hepatocellular carcinoma (LIHC) samples
We revealed that PCAT6 was a new CT‐long noncoding RNAs (lncRNAs) of LIHC
Its activation in LIHC tissues was associated with a poor prognosis and the knockdown of PCAT6 could significantly inhibit cell proliferation and migration both in HepG2 and SMMC‐7721 cells
Summary
State Key Program of National Natural Science of China, Grant/Award Number: 31530047; Science Fund for Creative Research Groups, Grant/Award Number: 81521004; National Natural Science Foundation of China, Grant/Award Number: 81602920, 81702266 and 81703295; National Key Basic Research Program, Grant/Award Number: 2015CB943003; China Postdoctoral Science Foundation, Grant/Award Number: 2017M610339 and 2018M630584; Jiangsu Planned Projects for Postdoctoral Research, Grant/Award Number: 1, 7, 0, 1041 and A; Top‐notch Academic Programs Project of Jiangsu Higher Education Institutions, Grant/Award Number: PPZY2015A067
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