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Abstract
Abstract An increase in chromatin template activity occurs within 1 hour after confluent monolayers of WI-38 human diploid fibroblasts are stimulated to proliferate by a change of medium. The present experiments were designed to investigate the role of non-histone chromosomal proteins in the stimulation of chromatin template activity. Chromatin from unstimulated WI-38 cells or from WI-38 cells 1 hour following stimulation was dissociated and then reconstituted with HeLa cell DNA, pooled histones, and non-histone chromosomal proteins from either unstimulated WI-38 cells or WI-38 cells 1 hour after stimulation. The template activity of chromatin reconstituted with non-histone chromosomal proteins from stimulated WI-38 cells was higher than the template activity of chromatin reconstituted with nonhistone chromosomal proteins from unstimulated cells. These results imply that the increased template activity of chromatin from stimulated WI-38 cells is due to changes in the constituent non-histone chromosomal proteins.
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