Abstract
Thednagenes, essential for protein priming DNA replication of bacteriophage φ29, are transcribed as a long polycistronic mRNA. In the previous study, gene 1 product (gp1) was shown to repress the expression of the upstreamdnagenes for DNA polymerase and primer protein. To investigate the details of the repression by gp1, we have examined the amount and integrity of polycistronic mRNA encoding DNA polymerase and primer protein by agarose gel electrophoresis and nuclease S1 protection assay. As a result, the amount, size, and integrity of the polycistronic mRNA were not influenced by the presence of gene 1. Furthermore, the RNA binding ability of gp1 was demonstrated by in vitro system using histidine-tagged gp1. These results strongly suggested that translation of thednagenes was affected by gp1 through binding to mRNA. Other possible mechanisms of gene regulation by gp1 were discussed.
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