Gender-related responses of dioecious plant Populus cathayana to AMF, drought and planting pattern

Abstract

In our previous studies, we detected drought, gender and arbuscular mycorrhizal (AM) inoculation effects on dioecious plant. Based on this, we investigated the intra- and inter-sexual competition between male and female plants. Dioecious plant Populus cathayana was used and we set 3 factors in this experiment: (1) AM inoculation/non-inoculation; (2) well-watered/water-stressed; (3) single-gender pattern (only 4 males or 4 females)/mixed-gender pattern (2 males and 2 females). Growth (stem length, ground diameter, SPAD, mean leaf area, biomass accumulation) and nutrition (C, N, P, K, Ca and Mg) distribution of male and female seedlings were determined. Results Drought significantly limited plant growth and nutrition accumulation, especially in female plants; AM formation alleviated this negative effect, especially in male plants. However, the gender effect was complicated. A mixed-gender planting pattern relieved intra- competition in terms of the growth and nutrient accumulation of both genders and even alleviated the negative effects caused by drought. In the mixed-gender pattern, the differences of C, N, P, K and Ca contents between male and female plants with AM inoculation was smaller than those without AM inoculation, which indicated a potential role for AM fungi in nutrient transport. Males had a stronger physiological response to limited water availability, and more advantages from AM formation than females. Mixed-gender planting relieved the existence of intra- sexual competition of dioecious plants, and AM symbiosis alleviated the differences between genders.