Gender specific association of missense variant rs1805097 of IRS-2 and noncoding variant rs841853 of GLUT-1 genes with susceptibility to type 2 diabetes in Bangladeshi population

Abstract

This study investigates occurrence of genotypic/allelic frequencies of missense rs1805097 and non-coding rs841853 variants within IRS-2 and GLUT-1 genes, respectively followed by their association with type 2 diabetes (T2D) in Bangladeshi population. Total 231 unrelated Bangladeshi (123 T2D and 108 healthy) individuals were included and genotypic distribution for both variants were validated using PCR-RFLP. Genotypic frequency distribution of the study participants followed Hardy Weinberg Equilibrium law. With respect to rs1805097 polymorphism, frequency of GG genotype was 1.23–fold lower while AG genotype frequency was 1.5–fold higher in T2D patients. The variant allele (A allele) of rs1805097 was significantly associated with the risk of T2D only in female, not male, individuals. For the rs841853 polymorphism, frequency distribution of GG genotype was lower while heterozygous genotype was higher in diabetic patients than healthy individuals (54.8% vs 62.04% and 41.74% vs 33.33%, respectively). Genotypic and allelic frequencies for rs841853 showed significant association with the risk of T2D only in female individuals. The genetic association for both these SNPs was non-significant after adjustment for key confounding factors. Moreover, we could not replicate the association of the rs1805097 within the IRS-2 gene with obesity. Further studies with increased sample size and appropriate controls for related confounding factors are needed to investigate these findings in an independent set of samples.