建構和評估Gellan gum/ Graphene薄膜作為傷口敷料之可行性

Abstract

In this study we prepared thick films of Gellan gum (GG)/ Graphene (GO) for wound-dressing applications. Gellan gum, an anion polysaccharide, it has biocompatibility and low production cost. Graphene, a single-atom-thick of carbon atoms, it possesses thermal, mechanical, and electrical properties. The applied film was fabricated using two different methods. The first method used of gellan gum mixed with graphene and reacted with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) to obtain a cross-linked film (GGM). The second method that of the prepared graphene is spray on gellan gum film, and then through EDC cross-linking to form the GGS film. The GGM with 63.97% gel content and 94.27% water content. The GGS with 60.82% gel content and 95.72% water content. Antimicrobial susceptibility test has resulted that GGM and GGS without effective antimicrobials for prevention and treatment of infections. Animal wound healing study, on day3, 7, 14, after surgery, the wound size reduction (%) in the GGM-treated group was 25.85%、45.18%、88.98%. On day3, 7, 14, after surgery, the wound size reduction (%) in the GGS-treated group was 20.85%、36.88%、75.38%. Thus, the GGM, GGS films developed in this study has potential for future use in surgical applications.