Gelatin/PVP/hydroxyapatite nanocomposite based on double micro-emulsion for tissue engineering applications

Abstract

Biomedical applications of composite hydrogels are attractive because of their characteristics. Gelatin/poly (vinyl pyrrolidone) (PVP) and HAp nanocomposite hydrogel are developed and characterized by FTIR, XRD, etc. In a bicomponent system, gelatin ensures biodegradability and biocompatibility. In contrast, PVP, a physiologic inert component, can impart elasticity to films and membranes due to its affinity for water and capacity to confer elasticity to films and membranes. Morphology, physicomechanical properties, water content, and in vitro studies were explored. To characterize the morphology of nanocomposite hydrogels, EDX and SEM were used. In addition, the Gelatin/PVP hydrogel composite had a water content of 85.8◦. Hydrophilicity was significantly enhanced by adding hydroxyapatite nanoparticles. Samples containing HAp showed the highest swelling ratios. Biocompatibility tests performed on L929 cells showed significant attachment and viability. Based on the results, Nanocomposite hydrogels might be suitable for tissue engineering because they support cell growth and proliferation well.