Abstract
The potential application of Pickering high-internal phase emulsions (HIPEs) in the food and pharmaceutical industries has yet to be fully developed. Herein, we synthesized fairly monodisperse, nontoxic, autofluorescent gelatin particles for use as sole stabilizers for fabricating oil-in-water (O/W) HIPEs in an effort to improve the protection and bioaccessibility of entrapped β-carotene. Our results showed that the concentration of gelatin particles determined the formation, microstructure, droplet size distribution, and digestion profile of the HIPEs. For storage stability, the retention of β-carotene in HIPEs was significantly higher than in dispersion in bulk oil, even after storage for 27 days. In addition, in vitro digestion experiments indicated that the bioaccessibility of β-carotene was improved 5-fold in HIPEs. This study will help establish a correlation between the physicochemical properties of gelatin particle-stabilized HIPEs with their applications in the oral delivery of bioactive nutraceuticals.
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