Gelatin-layered and multi-sized porous β-tricalcium phosphate for tissue engineering scaffold

Sung-Min Kim,Kwang-Mahn Kim,Yong-Keun Lee,Soon-Aei Yi,Seong-Ho Choi

doi:10.1186/1556-276x-7-78

Sung-Min Kim, Kwang-Mahn Kim + Show 3 more

Open Access

https://doi.org/10.1186/1556-276x-7-78

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Abstract

The multi-sized porous β-tricalcium phosphate scaffolds were fabricated by freeze drying followed by slurry coating using a multi-sized porous sponge as a template. Then, gelatin was dip coated on the multi-sized porous β-tricalcium phosphate scaffolds under vacuum. The mechanical and biological properties of the fabricated scaffolds were evaluated and compared to the uniformly sized porous scaffolds and scaffolds that were not coated by gelatin. The compressive strength was tested by a universal testing machine, and the cell viability and differentiation behavior were measured using a cell counting kit and alkaline phosphatase activity using the MC3T3-E1 cells. In comparison, the gelatin-coated multi-sized porous β-tricalcium phosphate scaffold showed enhanced compressive strength. After 14 days, the multi-sized pores were shown to affect cell differentiation, and gelatin coatings were shown to affect the cell viability and differentiation. The results of this study demonstrated that the multi-sized porous β-tricalcium phosphate scaffold coated by gelatin enhanced the mechanical and biological strengths.

Highlights

Tissue engineering is one of the important methods of constructing biological tissues or devices for reconstruction and repair of the organ structures in order to maintain and improve their function [1]
Characterization of the b-TCP scaffold Figure 1 shows the surface morphologies of the b-TCP scaffolds that were not coated by gelatin
The micro-CT results have shown that the TCP had a similar pore size at all of the cross-section area (Figure 2a), whereas MP had a macro-size pore in the middle of the b-TCP scaffolds (Figure 2b)

Summary

Introduction

Tissue engineering is one of the important methods of constructing biological tissues or devices for reconstruction and repair of the organ structures in order to maintain and improve their function [1]. The goal of scaffold production in tissue engineering is to fabricate reproducible, bioactive, and bioresorbable 3D scaffolds with appropriated properties that are able to maintain their structure for predictable times, even under load-bearing conditions [3]. The hydroxyapatite [HA], Ca10(PO4)6(OH), and b-tricalcium phosphate [bTCP], Ca3(PO4), are well-known bioceramics which are biocompatible and bioactive. These materials exhibit a close resemblance in chemical composition to the human

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