Abstract
Alaska Pollock is rich in resource. And during its processing, skin is peeled as byproducts. The skin of Alaska Pollock contains lots of collagen or gelatin, promising materials, which can be used in food, cosmetics, biomedical and pharmaceutical industries. Extracting collagen from the dry skin of Alaska Pollock is studied in this study to provide a better basement for fully using this renewable resource. Three kinds of gelatin were extracted by using acid, pepsin-assisted acid and hot water, respectively. The productivity of Acid Soluble Gelatin (ASG), Pepsin Soluble Gelatin (PSG) and hot Water Soluble Gelatin (WSG) were 7.33, 30.89 and 12.67%, respectively. Their UV absorption spectrum were similar and match with the absorption of dilute protein solution. The protein contents of ASG, PSG and WSG were 84-90%, with no significantly difference between them. The characters of SDS-PAGE and the circular dichroism spectrum shown that the PSG is broken into small fragment and has less secondary structure. In conclusion, the hot water extraction is the best way to extract the gelatin from the dry skin of Alaska Pollock.
Highlights
Alaska Pollock (Theragra chalcogramma) is the world's second most important fish species in terms of total catch (FAO, 2010)
Dry skin of Alaska Pollock was consisted by protein, water, lipid and the minerals and its major component is protein
The dry skin of Alaska Pollock is a good source of collagen
Summary
Alaska Pollock (Theragra chalcogramma) is the world's second most important fish species in terms of total catch (FAO, 2010). The Alaska Pollock has been said to be "the largest remaining source of palatable fish in the world" (Barowitz, 2009). During the processing of Alaska Pollock, skin are often peeled. These byproducts are discarded or used to produce low value feed (Guo et al, 2013). Extracting gelatin from the skin of Alaska Pollock improves the added value of low value byproduct, skin. As renewable biomaterials, gelatin is an important sustainable and harmless resource.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: American Journal of Biochemistry and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
