Abstract
Numerous recent studies have reported the identification of regulatory sequences located within either the 5′ or 3′ untranslated regions (UTRs) in individual mRNAs which are known to be controlled at the translational level (for review, see ref. ). It is generally presumed in these cases that the observed mRNA-specific translational regulation is mediated by protein(s) interacting with these sequences. The techniques of gel retardation (also known as band shift or gel mobility shift) and UV crosslinking have been particularly instrumental in the mitial studies to detect the putative regulatory factors.
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