Abstract
Abstract One of the most painstaking and time-consuming tasks involved in polyacrylamide disk electrophoresis is removing the gel from the glass tube. The problem becomes especially acute when high percentage (greater than 20%) acrylamide gels are used. Such gels are not only extremely stiff but also quite brittle and easily scratch or break. We have had success with a simple but highly efficient system for gel removal (Fig. 1). It could prove quite useful for laboratories dealing with large numbers of samples; perhaps even rapid enough for clinical use. A 18-mm × 6-mm vacuum hose 6 cm long is connected to the air outlet (which delivers approximately 50 psig) with a hose clamp. The other end of the hose is connected to an air valve. A second valve allows more precise control of the air pressure. A piece of similar vacuum tubing 10 cm in length is clamped to the opposite end of the second air valve. A 1/8-in. hole is drilled about 4 cm from the exposed end of this hose. A finger adjustable hose clamp is fitted over the exposed end.
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