Gel Permeation Chromatographic System: An Evaluation

Abstract

Abstract A gel permeation chromatography (GPC) system, including an automated GPC instrument, was evaluated and was found to be a reliable and efficient tool for fat-pesticide separations. In most cases more than 98% of the fat or oil was eluted from the column before the pesticides fraction began to elute. Cleanup efficiency was better than that obtained with acetonitrile partitioning, even when a petroleum ether backwash was used. Recoveries of several pesticides and industrial chemicals from milk fat were determined, with GPC as the only cleanup technique employed before gas chromatographic determination. Recoveries generally were better than those obtained with acetonitrile partitioning followed by Florisil cleanup. The automated instrument can be in operation unattended during non-working hours and, after loading, will automatically clean up 23 individual fat samples.