Abstract
Abstract: Introduction: Oviductus Ranae (OR) is the dried oviduct of female Rana temporaria chensinensis David, which is one of the best-known and highly valued oriental foods and medicines in China. OR has a unique physiological phenomenon that is high swelling capacity. Objectives: We aimed to study which components are associated with high swelling capacity in OR. Materials and Methods: isobaric tags for relative and absolute quantification (iTRAQ) proteomic methods were used to identify differentially expressed proteins between the OR and three counterfeit oviducts, including Rana nigromaculata, Bufo bufogargarizans and Rana catesbeiana to explore the mechanism of swelling capacity. Results: We found that a total of 1220 proteins were identified from 2149 unique peptide sequences. Comparing with three counterfeit oviducts, 11 differentially expressed proteins were identified in OR, including 9 up-regulated proteins, such as mucins (MUC2, MUC5AC, MUC5AC-like, MUC5B), PREDICTED: a-kinase anchor protein 13-like (AKAP13-like), PREDICTED: IgGFc-binding protein (FcγBP), PREDICTED: IgGFcbinding protein-like (FcγBP-like), et al. and 2 down-regulated proteins. Bioinformatics results found that these up-regulated proteins were involved in biological processes, including metabolic process, cellular process, responses to stimulus and biological regulation. Conclusion: Mucins, AKAP13-like, FcγBP and FcγBP-like may act as a positive regulator for the innate immune response in oviduct of Chinese brown frog and exceptional swelling capacity of OR is closely related to gel-forming mucins. Taken together, our results provide the basis for further understanding of the unique physiological phenomenon of OR. Key words: Oviductus Ranae, Swelling capacity, Gel-forming mucins, Counterfeit oviducts, Differentially expressed proteins.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Indian Journal of Pharmaceutical Education and Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.