Abstract
HELA human cancer cells have been shown to produce ferritin when grown in the presence of iron1. HeLa ferritin is electrophoretically heterogeneous; its properties have been compared with those of ferritin isolated from other cells grown in vitro and from human and animal tissues2. On DEAE chromatography of homogenates of HeLa cells grown in the presence of FeSO4 (59Fe), the distribution of the radioactivity was practically the same as the distribution of ultra-violet-absorbing components in the case of purified horse ferritin; the assignment of the activity to ferritin has been confirmed by paper electrophoresis3. The use of radio-iron in such experiments results in great sensitivity and specificity. We now report the behaviour of HeLa ferritin on gel-filtration.
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