Abstract
The gel-electrophoretic pattern of the proteoglycans of the tibial epiphyseal plate was studied in 7 foetuses, 5 premature newborns, 4 term newborns, 4 infants and 4 children. The proteoglycans obtained from small cartilage biopsies in cases with bone growth disturbances (achondroplasia; pseudo-achondroplasia, 3 cases; Kniest's disease, 2 cases; diastrophic dwarfism, 2 cases; parastrematic dwarfism; pycnodysostosis; mucolipidosis type III; Blount's disease and multiple exostoses) were compared with the proteoglycans of the normal cartilage.The tissues were extracted with 4 M Guanidinium chloride After dialysis against 8 M urea at pH 7, the proteoglycans were obtained by ion-chromatography in urea on D.E.A.E. cellulose and submitted to gel-electrophoresis on polyacrylamide-agarose gels.Gel-electrophoresis of proteoglycans showed a different pattern in foetuses from that found in children. In the groups studied, the change occurs in the first months of extrauterine life. In the pathological subjects studied, abnormal patterns were found in pseudo-achondroplasia and in Kniest's disease.
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