Gel Chromatographic Behavior of Inorganic Linear and Cyclic Phosphates: Influences of Eluting Agents

Abstract

Abstract Inorganic phosphates were chromatographed on a Sephadex G-25 column using lithium chloride, sodium chloride, potassium chloride, rubidium chloride, cesium chloride and tetramethylammonium chloride as an eluting agent at pH 10. When tetramethylammonium chloride was used the distribution coefficient of a linear phosphate was apparently smaller than that of the cyclic phosphate which has the same number of phosphorus atoms in its molecule. It was concluded that gel chromatographic behavior of phosphate species is greatly influenced by the nature of counter ions in an eluent.