Abstract
Early cancer detection using MR imaging is of high global interest as a powerful non-invasive modality. The aim of this study was to synthesize a nano-dendrimer and its conjugate with C595 mab against breast cancer cell, followed by its chelating with Gd3+. At the end, its ability as a nanosized probe for detection of breast cancer cells was investigated. For this reason, anti-MUC-1 mab C595 was coupled to a biocompatible anionic linear globular dendrimer G2 (ALGDG2) (Poly Ethylene Glycol PEG core and citric acid shell) followed by loading with Gd3+. MR imaging parameters of the prepared nanoconjugate was investigated in vitro performing several studies such as 1H-NMR and 17O-NMR, MUC-1 antigen. The obtained data showed a powerful relaxations as well as selective MUC-1 antigen and cell binding. Findings of this study showed that Gd3+-ALGDG2-C595 nano-probe is potentially, a selective breast molecular imaging agent.
Highlights
An important method for observing of nanosized contrast agents and their delivers into the tumor is selective imaging methods such as MR imaging for better targeting cancer
In MR imaging, contrast agent using nanoparticle plays an important role in this modality
Purification assay performed by RP-High Pressure Liquid Chromatography (HPLC) and the data shown a retention time of 7, 12 and 13 min for dendrimer, C595 alone and dendrimer-C595 conjugate, respectively with the purity of above 95% (Figure 1A)
Summary
An important method for observing of nanosized contrast agents and their delivers into the tumor is selective imaging methods such as MR imaging for better targeting cancer. Microsized cell entering carriers cannot traverse in a diffusion pattern through cells or pores including tumoric cells with pore sizes up to 380–780 nm. The best complex for tumor MR imaging applications would be a targeted nano-carrier complex [7,9,10,11]. Drug delivery using polymeric carriers is of global interest. In this regard, dendrimers (nanosized polymers) have been applied for delivering of contrast agents in MR imaging
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Biomolecular Research & Therapeutics
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
