GC\u2013MS analysis of phytocompounds and antihyperglycemic property of Hydrocotyle sibthorpioides Lam.

Abstract

Hydrocotyle sibthorpioides Lam. is a popular medicinal plant of Assam having several ethnomedicinal values. The present study investigated the metallic content, phytochemical contents, α-amylase, and α-glucosidase enzymes inhibitory property of H. sibthorpioides using in-vitro and in-silico methods. Heavy metal contents were analyzed using Atomic Absorption Spectroscopy. GC–MS was used to analyze the phytochemical compounds of the plant. Enzyme inhibition study was carried out by Spectrophotometry methods. The drug-likeness and toxicity properties of the phytocompounds were studied using SwissADME and ADMETlab databases. Docking and molecular visualizations were performed in AutoDock vina and Discovery studio tools. The study found that the extract of H. sibthorpioides contains a negligible amount of toxic elements. GC–MS analysis detected four compounds from the methanolic extract of the plant. Biochemical study showed considerable α-amylase and α-glucosidase enzyme inhibitory property of the crude extract of H. sibthorpioides. The IC50 of the plant extracts were found to be 1.27 mg/ml and 430.39 µg/ml for α-amylase and α-glucosidase enzymes, respectively. All four compounds were predicted to have potential drug-likeness properties with high cell membrane permeability, intestinal absorption, and less toxic effects. The docking study also showed strong binding affinities between the plant compounds and enzymes. Plant compound C2 showed an almost similar binding affinity with the α-amylase enzyme as compared to standard acarbose. The present study, thus, suggests the antihyperglycemic property of H. sibthorpioides and can be a potential source of antidiabetic drug candidates.