GCMS and FTIR spectral analysis of aqueous methylparathion biotransformation by the microbial mpd strains of Pseudomonas aeruginosa and Fusarium spp.

Abstract

This study aimed to find out the effective biotreatment processes of aqueous methyl parathion in stipulations of its biotransformation by potential indigenous mpd strains of Pseudomonas aeruginosa and Fusarium spp. Microbial biotransformation study of methyl parathion in contaminated synthetic wastewater at optimum conditions by P. aeruginosa mpd-5 (at temperature 33°C, pH 7, under the aerobic condition with inoculum density of 108cells/mL) and by Fusarium spp mpd-1 (at temperature 30°C, pH 7, under the aerobic condition with inoculum density of 25mgL-1 dry biomass) was carried out. The major biodegradation compounds formed during the biotreatment process were analyzed by spectral studies using GCMS and FTIR. GCMS analysis of bacterial transformed compounds was p-nitrophenol, dimethylaminophenol, and glycyl-L-proline of 2-(3-methylpiperidino-4-5, 6-benzothiazin-4-one) and 2, 5-piperazinodione 3, 6-bis (2-methylpropyl). And the fungal transformed compounds were observed to be p-nitrophenol, phenol,2,4-Bis(1,1-Dimethylethyl), beta-L-arabinopyranoside-methyl, Cyclobutanethiol, 3-2(4)-thiophenone, dihydro-5-(1-methylethyl), Benzene,1,3-bis(1,1-dimethyl ethyl), Butanoic acid, 2-methyl methyl ester, and L-(+)-Ascorbic acid. The FTIR spectra of the molecule and the products of methyl parathion are observed to be changed in structures. It can be concluded from the aforementioned results and discussions that P. aeruginosa mpd-5 and Fusarium spp mpd-1 can be used in the biotreatment of pesticide wastewater having the high methyl parathion concentration and is possible by the indigenous microbial strains; they utilize as phosphate and carbon source of energy. Hence the strains can be used as a whole microbial cell, or its bioactive metabolites can be applied for the biotreatment of pesticide wastewater and potentially degrade methylparathion.