Gc-Ms Analysis of Phytocostituents of Some Wild Zingiberaceae Plants Methanolic Rhizome Extracts

Abstract

Aim: To characterize the phytochemical constituents of Zingiber roseum,Curcuma angustifolia, Globba marantina using GC-MS technique. Methods: The crude extract form was subjected to column chromatography over silica gel (100-200 mesh) and eluted with n-hexane, chloroform, ethanol and methanol respectively. The methanol fractions of each plant were taken for GC-MS analysis. The component identification was achieved by the GC-MS analysis using HP 6890 series GC (Hewlett-Packard. USA) coupled with mass selective detection (MSD), HP5973 Series (Hewlett-Packard. USA).Helium was used as carrier gas and sample was injected in Split less mode in a column HP5. Phenyl methyl siloxane (25μl (film thickness) ×320μm (internal diameter×30 m (length of the column). Mass spectra were acquired over 40-400 atomic mass unit range. Temperature programming: Initial temperature -60°C. Ramping rate -3o/min. Final temperature-243°C. Run time-61 min. Results: The GC-MS analysis was provided different peaks of seventeen compounds of Zingiber roseum, fifteen of Curcuma angustifolia, and nine of Globba marantina, Zingiber roseum has Deoxynivalenol (0.43%), n-heptane (0.74%), Cyclopentane,1-methyl-2 (2-propyl) trans-(2.76%), Ethyl propionate (0.24%), Ethyl heptanoate (0.21%), Naphazoline (0.76%), Estazolam (3.20%), 2-hexane (20.00%), 3-Heptanone (29.29%), 1,4-butanediol (0.93%), Diehylene glycol methyl ether (0.20%), Iso-propyl benzene (Cumene) (4.47%), Isoprene (0.34%), Propofol (0.51%), Pentadecenol (18.08%), n-Amylmercaptan (8.65%), Methyl cyclopentane (9.20%), Curcuma angustifolia has 3-hydroxyhexanoic acid (0.51%),α –thujene (1.41%), β- pinene (1.67%), Cis-p-menth-2,8-dienol (2.14%), 3,5-dimethoxy (Tolune) (1.19%), Caryophyllene (1.39), Flurprimidol (2.61%), 2,7-napthalenediol (12.44%), Camphor (15.38%), Trans-nerolidol (12.02%), Humulen-6,7-epoxide (9.21%), Octadecanoic acid, butyl ester (11.56%), α-amorphene (23.02%), Aristoloshene (3.80%), Diflobenzuran (1.63%), Globba marantina has Heptadecane (6.19%), Pinocarvone (54.27%), L-linalool (2.54%), Alloaromadendrene (4.58%), β –caryophyllene (13.65%), α- humulene (3.88%),Terpineol (6.31%),Lavandulol (2.63%),2,6-Dimethyl-1,5,7-Octatrien-3-ol (5.95%) .Conclusions: Bioactive compounds found in Zingiber roseum, Curcuma angustifolia, Globba marantina has been screened using GC-MS analysis this isolation various compound may helps vital role to find new drugs.