GC-MS Analysis of Phytochemicals and Simultaneous Determination of Flavonoids in Amaranthus caudatus (Sirukeerai) by RP-HPLC

Abstract

A sensitive and selective high performance liquid chromatography method (HPLC) for simultaneous analysis of following five flavonoids like Gallic acid (GA), Caffeic acid (CA), Rutin (RU), Ferulic acid (FA) and Quercetin (QU) in Amaranthus caudatus (Sirukeerai) leaves. The results demonstrated that the Amaranthus caudatus leaves were separately extracted and analyzed using RP HPLC method. The contents of Gallic acid (0.083 μg/gm), Caffeic acid (0.004 μg/gm), Rutin (0.019 μg/gm), Quercetin (0.001 μg/gm) and Ferulic acid (0.001 μg/gm) in Amaranthus caudatus leaves. The GC-MS study also carried out and it showed the presence of phytochemicals. Hence these plants extracts with the property of bioavailability and retention of certain minerals by polyphenolic compounds can be recommended for their use as an alternative anti-infective agent in natural medicine for the treatment of infectious diseases.