GC-MS Analysis of extract for Endophytic fungus Acremonium coenophialum and its Antimicrobial and Antidiabetic

Abstract

The aim of the present work was to study antimicrobial and antidiabetic activities of the fungal extract of endophytic fungus Acremonium coenophialum isolated from medicinal plant Myrtus communis was evaluated. The fungal extract was carried out by Ethyl acetate solvent. Column Chromatography, Thin Layer Chromatography (TLC) and Gas chromatography-mass spectrometry (GC-MSS) were used to determine the chemical composition of fungal extract from endophytic fungus A. coenophialum. As a result, a total of nineteen compounds of Fungal extract were demonstrated for antimicrobial activity against seven Gram-Positive Bacteria named Staphylococcus aureus, S. epidermidis, Streptococcus Pneumoniae, Strep. pyogenes, Enterococcus faecalis, Bacillus cereus and B. subtilis which range between (10.0-17.0) mm against seven Gram-Negative Bacteria namely; Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Morganella morganii, Serratia sp. and Proteus vulgaris which range between (10.0-18.0) mm and against both yeasts test was (10.0) mm. Minimum inhibitory concentrations were in the range between (25.0-100), (50-100)µg/ml and (100)µg/ml against Gram-Positive bacteria, Gram-Negative and yeasts test respectively. Minimal bactericidal concentrations ranged from (50-100)µg/ml and (100-200)µg/ ml against Gram-Positive bacteria and Gram-Negative bacteria respectively and minimal fungicidal concentrations (200)µg/ml against both yeasts test. The fungal extract also exhibited antidiabetic potential by inhibition of a-amylase activity. The activity was found to be similar to that of acarbose.