GCMS Analysis of Bioactives of Piper betle Linn. Leaf

Abstract

Background: Piper betle Linn. (Piperaceae) is commonly known as “Green gold of India” due to its high commercial value. It is a Vedic plant used in various traditional system of medicine v.iz. Ayurveda system of medicine, Yunani system of medicine attributed to various pharmacological activities v.i.z. antioxidant, anti-inflammatory, analgesic, anti-fertility, antimutagenic etc. Objectives: The main objective of the present study is quantitative estimation of the various bioactive compounds present in leaf of P. betle L. (Bangla variety) by GC-MS analysis using head space method. Material and Methods: The vapor sample of leaf of P. betle L. was prepared by head space method for GCMS analysis. GCMS analysis was carried out using high resolution Thermo scientific TSQ 8000 Gas Chromatograph-Mass Spectrometer. Helium is used as carrier gas (99.999 %) with a constant flow rate of 1 ml/min. Total run time was 29.10 min. Results: GC-MS analysis revealed the presence of 16 bioactive compounds in leaf of P. betle L. Eugenol (29.13%), Bakuchiol (11.79%) and α-linalool (14.88%) are the chief bioactive compounds of leaf. Leaf has been also reported to contain a good percentage of various terpenoids like β- caryophyllene (7.69), α-muurolene (4.19), α-bisabolene (0.98), etc. Conclusion: From GC-MS analysis results it can be concluded that leaf of P. betle L. is a reservoir of valuable phenolic and susquiterpene compounds. Eugenol chief biomarker compound present leaf is a versatile remarkable molecule. Due to number of phytoconstituents P. betle L. leaf is recommended as plant of phytopharmaceutical importance.