Abstract
Objective: To investigate the photochemical constituents present in methanol extract of martynia annua seeds using Gas Chromatography-Mass Spectroscopy(GC-MS), High-Performance Thin–Layer Chromatography(HPTLC) analysis and study antioxidant activity.
 Methods: Methanol extract of Martynia annua seeds were subjected to GC-MS and HPTLC analysis. HPTLC analysis was carried out using GAMAG system with a linomate5 applicator, system mobile phase (Toluene: Chloroform: Ethanol (4:4:1 V/V/V)), two different volume of extract was applied 2 µl and 5 µl. GC-MS analysis was carried out on JEOL GC MATE ΙΙ, column HP 5 MS and Quadruple double focusing mass analyzer. Antioxidant activity was determined by DPPH assay.
 Results: GC-MS analysis provided 17 peaks indicating the presence of seventeen different phytochemicals in methanol extract of martynia annua seeds. HPTLC fingerprint showed 6 peaks at both size 2 µl and 5 µl at 254 nm whereas 4 peaks, 9 peaks were detected at 366 nm for 2 µl and 5 µl respectively. After derivatization with 10 % methanolic sulphuric acid, 8 peaks, 11 peaks were detected for 2 µl and 5 µl respectively when the derivatized plate was scanned at 540 nm. DPPH free radical scavenging result showed EC50 value of 44.1±1.1 µg/ml.
 Conclusion: The GC-MS analysis showed the presence of fatty acids, ester, aldehydes and ketones whereas in HPTLC different peaks at different UV-lights before and after derivatization were observed. Maximum percentage inhibition using DPPH assay was found 74 at concentration of 50 µg/ml.
Highlights
The goals of using plants as source of therapeutic agents to use the hole plant or part of it as a herbal remedy and the bioactive compounds can be isolated from the plant for direct use as drugs, to use agents as pharmacological tools, to produce bioactive compounds of new or known structures as model compounds for semi-synthesis to synthesis patentable entities of higher activity and/or lower toxicity [1]
Martynia annua belong to Martyniaceae family [3], it is known as Devil's Claw [4]
Standardization of plant material is need of the day [12] but the standardization and quality control of herbal drugs is very complicated due to variation of phytoconstituents within the same plant or from part to part of same plant [13]. Modern chromatographic techniques such as GCMS and High performance thin–layer chromatography (HPTLC) fingerprint play an important role for identification, quantification of the chemical composition of plant material and may be useful for proper standardization of herbal drug formulation [14,15,16]
Summary
The goals of using plants as source of therapeutic agents to use the hole plant or part of it as a herbal remedy and the bioactive compounds can be isolated from the plant for direct use as drugs, to use agents as pharmacological tools, to produce bioactive compounds of new or known structures as model compounds for semi-synthesis to synthesis patentable entities of higher activity and/or lower toxicity [1]. Martynia annua belong to Martyniaceae family [3], it is known as Devil's Claw [4]. It is from Mexico natively [5] and found in throughout India [6]. Standardization of plant material is need of the day [12] but the standardization and quality control of herbal drugs is very complicated due to variation of phytoconstituents within the same plant or from part to part of same plant [13]. The aim of this study was to investigate the phytochemical constituents present in methanol extract of martynia annua seeds using GC-MS and HPTLC analysis and evaluation of antioxidant activity using DPPH assay
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