GCMS analysis and invitro antibacterial and anti-inflammatory study on methanolic extract of Thalassiosira weissflogii

Abstract

Abstract Microalgae possess huge kind of natural bioactive compounds with potent values. In the present study, analysis of invitro anti inflammatory and anti bacterial activity from Thalassiosira weissflogii and anti inflammatory activity of extract was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assay. Apart from this the methanolic extract of diatom T. weissflogii was subjected to antibacterial activity against different organisms such as E. coli, S. aureus and B. subtilis by agar well diffusion method. T. weissflogii against E. coli, S. aureus and B. subtilis was found to be 12 mm, 19 mm and 17 mm respectively. This was compared with inhibitory zone showed by 30 μg/ml of streptomycin that exhibit maximum of 18.6 mm for E. coli, 15.2 mm for S. aureus and 26 mm for B. subtilis. We found to contain various chemical constituents such as pigment, phenolic, tocopherol. The secondary metabolites of Diatom, Thalassiosira weissflogii was analysed and characterised using various analytical techniques such as UV–vis Spectroscopy and GCMS. In the present study, methanolic crude extract of T. weissflogii was extracted and examined for in vitro anti-inflammatory and antimicrobial activity. The future work will be determination of anti inflammatory activities by in vivo models.