Abstract
Aim: This study aimed to develop and validate a GC-NPD method for quantifying topiramate (TPM) in capillary dried plasma spots (DPS).Materials & methods: Extraction involved three 6mm DPS with albumin 0.1%, alkaline liquid extraction with tert-Butyl methyl ether and TMAH methylation. The method was validated and applied to 15 paired samples of capillary DPS and venous plasma from chemical dependency patients.Results: The method was linear from 1 to 50μg/ml (r>0.99), precise (CV% 3.62-8.29%)accurate (98.1-107.7%). TPM stability was confirmed in DPS stored at 4, 23 and 45°C for 21days. DPS TPM measurements were highly correlated plasma concentrations (rs=0.96), representing on average 102% of the venous plasma measurements.Conclusion: The method was fully validated, demonstrating potential for clinical application.
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