GC-MS Profiling of Anti-bacterial Metabolic Compounds from the Extract of Azadirachta indica

Abstract

Azadirachta indica is a very common plant used very frequently due to its medicinal significance. The antibacterial activities of 0.001, 0.01, 0.1, 1.0 and 10 mg/mL of the plant extract were determined against different pathogenic bacteria. Concentration of 0.01 mg/mL killed the E. coli, E. aerogenes, P. stuartii and 10, 1.0 and 0.1 mg/mL were very effective against the E. cloacae, K. pneumoniae and P. mirabilis and killed them 100% in culture plates. The plant extracts were analyzed for the characterization of the different antimicrobial compounds through gas chromatography-mass spectrometry (GC-MS). An array of antibacterial compounds such as azulene, tetrasiloxane, phthalic acid, cyclopentasiloxane, hexadecanoic acid, spiropentane, dioctyl phthalate were detected in the plant extract through GC-MS. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as antimicrobial compounds.