GC/MS profile, DNA protectant and hepatoprotective effects of Praecitrullus fistulosus fruit methanol extract.

Abstract

Abstract The aim of the present investigation is to evaluate the antioxidant, DNA protectant and hepatoprotective effects of methanol extract of Praecitrullus fistulosus fruits. The antioxidant and DNA protectant activity using in vitro model and hepatoprotective effect using in vivo model was assessed. Antioxidant potential of MeOH was assessed by ABTS, hydroxyl radical, FRAP and H2O2 scavenging activity. Further cell-based antioxidant activity was determined using RBC's cells. Hepatoprotective effects of MeOH were assessed using CCl4 induced liver damage mice model, in vivo. Post experimental results have proven the protective role of MeOH extract on the liver was assessed by serum antioxidant and biochemical analysis followed by histopathological studies. Histopathological studies reveal the retained tissue architecture, decreases hepatocellular necrosis, a poor dilated central vein in MeOH treated group. Further in vivo antioxidant enzyme activity showed that MeOH significantly protect the liver injury by retaining the antioxidant enzyme level in the CCl4 treated groups. This study provides preliminary evidence that the edible fruits Praecitrullus fistulosus as nutraceuticals in food formulation and product development in the pharmaceutical industries.