Abstract
Background and objectives: Natural products such as essential oils with antioxidant potential can reduce the level of oxidative stress and prevent the oxidation of biomolecules. In the present study, we investigated the antioxidant potential of Lantana montevidensis leaf essential oil (EOLM) in chemical and biological models using Drosophila melanogaster. Materials and methods: in addition, the chemical components of the oil were identified and quantified by gas chromatography coupled to mass spectrometry (GC-MS), and the percentage compositions were obtained from electronic integration measurements using flame ionization detection (FID). Results: our results demonstrated that EOLM is rich in terpenes with Germacrene-D (31.27%) and β-caryophyllene (28.15%) as the major components. EOLM (0.12–0.48 g/mL) was ineffective in scavenging DPPH radical, and chelating Fe(II), but showed reducing activity at 0.24 g/mL and 0.48 g/mL. In in vivo studies, exposure of D. melanogaster to EOLM (0.12–0.48 g/mL) for 5 h resulted in 10% mortality; no change in oxidative stress parameters such as total thiol, non-protein thiol, and malondialdehyde contents, in comparison to control (p > 0.05). Conclusions: taken together, our results indicate EOLM may not be toxic at the concentrations tested, and thus may not be suitable for the development of new botanical insecticides, such as fumigants or spray-type control agents against Drosophila melanogaster.
Highlights
Reactive species are known to cause damage to cellular membranes, DNA, proteins, and mitochondria, and have been found to be involved in the pathophysiology of a wide rangeMedicina 2019, 55, 194; doi:10.3390/medicina55050194 www.mdpi.com/journal/medicinaMedicina 2019, 55, 194 of diseases such as brain ischemia, carcinogenesis, diabetes, etc. [1,2]
Considering that antioxidant activity has been reported for L. montevidensis [9], we investigated the effects of essential oil of L. montevidensis (EOLM) on the indirect biomarker of oxidative stress, total thiol
The results obtained in this study showed that EOLM at all the concentrations tested did not alter the total thiol and NPSH levels and the MDA content of flies homogenates, indicating that EOLM does not cause oxidative stress since the total thiol depletion is associated with increased lipid peroxidation [65]
Summary
Reactive species are known to cause damage to cellular membranes (lipid peroxidation), DNA, proteins, and mitochondria, and have been found to be involved in the pathophysiology of a wide rangeMedicina 2019, 55, 194; doi:10.3390/medicina55050194 www.mdpi.com/journal/medicinaMedicina 2019, 55, 194 of diseases such as brain ischemia, carcinogenesis, diabetes, etc. [1,2]. Leaf extract of L. montevidensis was shown to exhibit antioxidant activity [9], while flavonoids from its leaves were reported to exert antiproliferative activity against gastric adenocarcinoma, human uterus carcinoma, and murine melanoma cells, in vitro [21]. Natural products such as essential oils with antioxidant potential can reduce the level of oxidative stress and prevent the oxidation of biomolecules.
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